Figure 2.

Characterization of the Effects of the ID-Associated Mutation on OGT TPR Stability and Dynamics

(A) Thermal denaturing curve showing fraction of unfolded TPR_WT and TPR_L254F constructs as a function of temperature. Data averaged from seven replicates were fitted to a Boltzmann sigmoidal curve equation, with error bars representing SD.

(B) Superposition of sTPR_WT (gray), sTPR_L254F-C1 (green), and sTPR_L254F-C2 (purple), with the B-A′-B′ angle and intra-TPR distance demarcated with solid and dashed lines, respectively. See also Figures S2 and S3.

(C) Graphs of sTPR_WT (left) and sTPR_L254F (right) conformational populations in the molecular dynamics simulations, with the χ₁ dihedral angle of residue 254 shown on the x axis, the intra-TPR repeat distance shown on the y axis, and the angle B-A′-B′ shown as a color scale. The B-A′-B′ values observed in the crystal structures are shown as black dots. Histograms attached to the graph show the distribution of χ₁ dihedral angles. See also Figures S2 and S3.

sTPR_L254F-C1 and sTPR_L254F-C1, sTPR_L254F conformations 1 and 2; sTPR, simplified TPR; TPR, tetratricopeptide repeats.