REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti O-GlcNAc (RL2)	Abcam	ab2739; RRID:AB_303264
Rabbit polyclonal anti HSP90	Cell Signalling	Cat#4874; RRID:AB_2121214
Rabbit polyclonal anti HSP90	Enzo Life Sciences	ADI-SPA-836-D; RRID:AB_991589
Rabbit polyclonal anti OGT (H-300)	Santa Cruz Biotechnology	sc-32921
Goat IRDye® 680RD anti-Mouse	LICOR	P/N 925-68070; RRID:AB_621840
Donkey IRDye® 800CW anti-Rabbit	LICOR	P/N 925-32213; RRID:AB_621848
Morpheus® HT-96 Crystal Screen	Molecular Dimensions	MD1-47
Bacterial and Virus Strains
E. coli BL21-DE3	New England Biotechnologies	C25271
Chemicals, Peptides, and Recombinant Proteins
SYPRO® Orange dye	Sigma	S5692-50UL
GlcNAcstatin-G (OGA inhibitor)	GlycoBioChem	GBC10002
RB2 (residues 410-422)	GlycoBioChem	N/A
CpOGA	GlycoBioChem	N/A
OGTWT/L254F	This paper	N/A
TPRWT/L254F	This paper	N/A
Deposited Data
TPRL254F structure	This paper	PDB: 6EOU
Experimental Models: Cell Lines
HEK-293	ATCC	CRL-1573
Oligonucleotides
Primer TPR: Forward CTGGGATCCGGCCCGATGGAACT GGGCTCATCGTGAAATATCAG	This paper	N/A
Primer TPR: Reverse GATGCGGCCGCTTAGTCTTGCATT TCTTTCAGCGTATTAC	This paper	N/A
Recombinant DNA
pGEX-6P-1 Vector	GE Healthcare	28954648
pHEX-6P-1 Vector	This paper	N/A
Software and Algorithms
Graphpad Prism v5.0	GraphPad Software	https://www.graphpad.com/
iMosflm	(Battye et al., 2011)	http://www.ccp4.ac.uk/
CCP4	(Winn et al., 2011)	http://www.ccp4.ac.uk/
REFMAC	(Vagin et al., 2004),	http://www.ccp4.ac.uk/
GROMACS	(Abraham et al., 2015).	http://www.gromacs.org/
MDAnalysis	(Michaud-Agrawal et al., 2011)	https://www.mdanalysis.org/
MDTraj	(McGibbon et al., 2015)	http://mdtraj.org/1.9.0/
Other
Morpheus® HT-96 Crystal Screen	Molecular Dimensions	MD1-47