Fig. 5.

Comparison of the reactivation profile of the deprived visual cortex in Stim-7wME (red) and DE-Stim-7wME mice (blue) versus Sham-7wME mice (black). Line graphs illustrate the relative zif268-mRNA expression level measured as the average OD-value per segment (0–24) from lateral to medial for sham-7wME mice, Stim-7wME mice and DE-Stim-7wME mice. The expression levels are displayed in relation to the visual subdivisions separated by hollow arrowheads which are in accordance with the small arrowheads in Fig. 4 a–d, including the subdivision between monocular zones m and binocular zones b, separately for supra- and granular layers II/III and IV (a) and infragranular layers V and VI (b). Error bars represent SEM. Relative zif268-mRNA expression levels are displayed as OD-values averaged over the binocular zone, Bz and the medial monocular zone, Mmz for upper (c) and lower layers (d). Bz corresponds to V2Lb combined with V1b (dark grey) and Mmz to V1m and V2M (light grey) as displayed in a, b. Error bars are SEM. Comparison of the OD-values of sham-7wME mice and Stim-7wME mice to DE-Stim-7wME mice indicates severely lowered reactivation levels across all cortical zones and layers for stimulated and DE-Stim animals (sham-7wME, upper layers, Bz: Average ± SEM; 85.87 ± 0.99; Mmz: 69.48 ± 1.90; lower layers, Bz: 81.29 ± 0.76; Mmz: 69.72 ± 1.09; Stim-7wME, upper layers, Bz: 73.83 ± 1.71; Mmz: 63.99 ± 1.91; lower layers, Bz: 69.00 ± 1.37; Mmz: 58.33 ± 2.17; DE-Stim-7wME, upper layers, Bz: 63.99 ± 1.91 ; Mmz: 34.72 ± 1.90; lower layers, Bz: 58.57 ± 1.61; Mmz: 40.78 ± 1.14). *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001