Table 1.
Antibodies used
| Antigen | Host | Source | Code | Dilution |
|---|---|---|---|---|
| Primary | ||||
| PV | Mouse | Swant | 235 | 1:3000 |
| PV | Rabbit | Swant | PV27 | 1:3000 |
| CR | Rabbit | Swant | CR7697 | 1:500 |
| CR | Mouse | Swant | 6B3 | 1:1000 |
| CB D-28 k | Mouse | Swant | 300 | 1:2000 |
| CB D-28 k | Rabbit | Millipore | AB1778 | 1:200 |
| ChAT | Goat | Chemicon | AB144P | 1:150 |
| Islet1 | Mouse | DSHB | 40.2D6 | 1:100 |
| GABA | Rabbit | Sigma | A2052 | 1:2000 |
| Glycine | Rat | ImmunoSolution | IG1002 | 1:1000 |
| Glutamate | Rabbit | Sigma | G6642 | 1:4000 |
| Serotonin | Rabbit | Sigma | S5545 | 1:4000 |
| Sox-2 | Goat | R&D Systems | AF2018 | 1:500 |
| Elav3 + 4 (HuC/D) | Rabbit | GeneTex | GTX128365 | 1:500 |
| Secondary | ||||
| Goat IgG-568 | Donkey | ThermoFisher | A-11057 | 1:500 |
| Mouse IgG-647 | Donkey | ThermoFisher | A-31571 | 1:500 |
| Mouse IgG-568 | Goat | ThermoFisher | A-11004 | 1:500 |
| Mouse IgG-488 | Donkey | ThermoFisher | A-21202 | 1:500 |
| Rat IgG-550 | Donkey | ThermoFisher | SA5-10027 | 1:500 |
| Rabbit IgG-488 | Donkey | ThermoFisher | A-21206 | 1:500 |
| Rabbit IgG-568 | Donkey | ThermoFisher | A-10042 | 1:500 |
CB calbindin D-28 k, CR calretinin, PV parvalbumin, ChAT choline-acetyltransferase, GABA γ-Aminobutyric acid