Figure 3.

Inhibition of RyR3 by ryanodine suppresses OPC differentiation. (A) Ca²⁺ recording of OPCs in proliferation medium (Pro-medium) showing random spontaneous waves. (B) OPCs cultured in differentiation medium (Diff-medium) for 6 h showed frequently higher peak waves, which could be inhibited by ryanodine (Rya) (C). (F) The total amount of Ca²⁺ release, reflected by the area under curve, was greater in early differentiating OPCs than in proliferating OPCs (**p < 0.05), and the release activity in early differentiating OPCs was greatly reduced by ryanodine (**p < 0.05). (D,E) There were fewer MBP-positive mature OLs after treatment with ryanodine (50 μM) for 72 h. Scale bar 50 μm. (G,H) Western blot analysis showed the same inhibitory effect of ryanodine on OPC differentiation (**p < 0.05).