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. 2018 May 18;12:133. doi: 10.3389/fncel.2018.00133

Figure 8.

Figure 8

Expression of cFos immunoreactive cells in the lumbosacral spinal cord. (A) Coronal diagram of the rat lumbosacral spinal cord (L6) showing the location of the dorsal horn (i.e., laminae I and II), the sacral parasympathetic nucleus and the dorsal commissure (Rat brain atlas, Paxinos and Watson, 2007) and a corresponding photomicrograph showing a spinal cord section stained for cFos of stimulated rat with bipolar stimulation electrode implanted in the bladder wall in the D-N configuration. The higher power photomicrograph in the right corner shows the dorsal horn of the spinal cord. Abbreviations: I–II: laminae I and II, DCM: dorsal commissure, SPN: sacral parasympathetic nucleus. Scale bar = 200 μm. Cumulative data showing the means and SEM for the optical density of cFos expression for D-N group in the (B) SPN, (C) DCM and (D) I–II of L6-S2 spinal cord. Control n = 4, Sham D-N n = 4 and Stim D-N n = 4. t-test, *p < 0.05, **p < 0.005 and ***p < 0.001.