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. 2018 Apr 20;38(2):BSR20171193. doi: 10.1042/BSR20171193

Figure 2. SFTA1P inhibits GC cell proliferation in vitro.

Figure 2

(A) qRT-PCR analysis of SFTA1P expression in the normal gastric epithelium cell line (GES1) and GC cells. (B) qRT-PCR analysis of SFTA1P expression in empty vector, pCDNA-SFTA1P transfected GC cells. (C,D) MTT assays were used to determine the viability of pCDNA-SFTA1P transfected GC cells. (E) Colony formation assays were performed to determine the proliferation of pCDNA-SFTA1P transfected GC cells. Colonies were counted and captured. (F,G) Proliferating BGC823 and SGC7901 cells were labeled with EdU. The Click-it reaction revealed EdU staining (red). Cell nuclei were stained with DAPI (blue). Representative images and data based on three independent experiments. Error bars indicate mean ± S.E.M. *P-value <0.05, **P-value <0.01.