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. 2018 May 18;9:577. doi: 10.3389/fpls.2018.00577

FIGURE 1.

FIGURE 1

(A) Schematic representation of T-DNA insertion (+480 bp from the translational start site) at Os01g02290 encoding rrsRLK in Dongjin (ΔrrsRLKdj). ATG start codon and TGA stop codon are indicated. Os01g02290 consists of four exons (black boxes) and three introns (line between the black boxes). (B) Expression analysis of the rrsRLK gene in SE-WTdj (segregated wild type) and ΔrrsRLKdj mutant lines using RT-PCR with rrsRLK primers. Ubi gene was used as an internal control. The experiment was repeated three times with consistent results. (C) Comparison of lesion development on leaves of SE-WTdj and homozygous mutant lines of ΔrrsRLKdj. Rice plants grown for 6 weeks were inoculated with PXO99 and monitored for lesion development for 14 DAI. The results were consistent until the 6th generation of the mutant, and each experiment was carried out with more than 40 leaves. (D) Lesion lengths scored at 14 DAI on leaves of SE-WTdj and ΔrrsRLKdj mutant lines. The averages and error ranges of each sample were calculated from 40 leaves. Asterisk indicates P < 0.05 (Student’s t-test). Data in (C,D) are results of experiments carried out using the 3rd generation of ΔrrsRLKdj mutant lines. (E) Bacterial population extracted from inoculated leaves of SE-WTdj and ΔrrsRLKdj mutant lines at 0 and 14 DAI. Xoo was extracted from inoculated leaves and incubated on PSA plates after serial dilution for colony counting. Each data point represents the average and standard deviation of three biological replicates. The experiment was repeated three times with similar results. Different letters above bars indicate statistically significant differences as determined by one-way analysis of variance (ANOVA: ∗, a, b, c), P < 0.05.