FIGURE 4.

Sub-cellular localization of the rrsRLK protein in rice cells. The rrsRLK-GFP fusion protein was transiently expressed in OC cells and monitored with a confocal microscope equipped with filter sets for GFP (excitation wavelength/dichroic transition: 488/543 nm) and RFP (excitation wavelength/dichroic transition: 561/575 nm). GFP, OC cells showing rrsRLK-GFP green fluorescence in cytosol; RFP, the same cell showing plasma membrane and nuclear localization using PM-mRFP and NLS-mRFP markers (Kim et al., 2009); Bright field, a differential interference contrast image; Merged, the merged image of GFP and RFP.