FIGURE 7.

(A) H₂O₂ content in tissues of SE-WT_dj and ΔrrsRLK_dj. Content of H₂O₂ in 1 g of tissue (root, stem, and leaf) of SE-WT_dj and ΔrrsRLK_dj was spectrophotometrically determined at 436 nm by the modified method of Bernt and Bergmeyer (1974) using peroxidase enzyme. (B) Activity assay of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POX) in leaves of SE-WT_dj and ΔrrsRLK_dj. Total protein was extracted from 1 g of leaves from SE-WT_dj and ΔrrsRLK_dj rice, and the activity of ROS-scavenging enzymes was spectrophotometrically monitored at A_560nm (SOD), A_240nm (CAT), A_290nm (APX), and A_436nm (POX) using a modified method of Beyer and Fridovich (Beyer and Fridovich, 1987). All assays were repeated more than three times, and the error bars of each sample denote the mean ± SD (n = 9). The asterisks indicate that a significant difference (P < 0.05) in the H₂O₂ was detected between transgenic plants and wild type.