Figure 1.
Evaluation of proinflammatory cytokines in RAW cells stimulated with GAS or E. coli. RAW264.7 cells were seeded at a concentration of 1 × 106/well in a 6-well plate containing medium without antibiotics overnight and then infected with GAS, or E. coli as a control, at an MOI of 10. We then collected supernatant and cells from each group at different time points. Total RNA was isolated from cells using RNeasy, and qPCR analysis was performed to detect the mRNA level of IL-1β (A), TNF-α (B), and IL-6 (C). Simultaneously, ELISA was performed to detect the protein expression of IL-1β (A′), TNF-α (B′), and IL-6 (C′) in the cell supernatant. Each experiment was performed at least three times. Results are presented as mean ± SD. P < 0.05 vs. E. coli group.