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. 2018 May 11;8:131. doi: 10.3389/fcimb.2018.00131

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Copyright © 2018 Ma, Gao, Wu, Zhang, Wang, Zhang, Yao, Song, Li, Wang, Feng and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological evaluation of inflammatory response in mouse lung tissue following GAS or E. coli infection. The three experimental groups of mice were separately prepared for histopathological analysis. (A) Representative views of lung sections treated with PBS, GAS, or E. coli. In the left panels, H&E staining shows the overall outline of lung slices, with a magnified image. Inflammatory cells are identified by red circles in the middle panels (H&E staining, × 10 and ×40). Periodic acid–Schiff (PAS) staining showed bronchiolar mucus production in each group (right panels, objective ×20). (B) Quantitative analysis of recruited inflammatory cells per square mm. (C) Inflammatory cells were counted in lung lavage fluid (per ml). Each experiment was repeated three times, and similar results were obtained on each occasion.