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Expression of proinflammatory cytokines in RAW 264.7 macrophages with and without A20 SiRNA following GAS infection. RAW 264.7 cells were incubated with A20 SiRNA for 30 min, and then infected with GAS at an MOI of 10. And 6 h post-infection, ELISA and RT-PCR were performed to analyze the expression of proinflammatory cytokines. Each experiment was repeated three times, and similar results were obtained on each occasion. Results are presented as mean ± SD.
