Figure 7.

WT RAW cells induced by gene mutant strain M^−/− or SpeB^−/− GAS, or WT GAS, or purified M protein. RAW cells were stimulated by WT or M^−/− (A) or SpeB^−/−GAS (B) at an MOI of 10. Six hours later, the treated cells were collected and A20 expression was determined by Western blotting. ^*P < 0.05 vs. the M^−/− GAS group. RAW cells were stimulated by recombinant M-GST protein, GST-tag alone, and PBS as a control (C), or recombinant purified M protein and PBS as a control (D). Six hours later, the treated cells were collected and A20 expression was determined by Western blotting. Each experiment was repeated three times, and similar results were obtained on each occasion.