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. 2018 May 18;9:500. doi: 10.3389/fphar.2018.00500

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Copyright © 2018 Ulrich, Ratajczak, Schneider, Adinolfi, Orioli, Ferrazoli, Glaser, Corrêa-Velloso, Martins, Coutinho, Santos, Pillat, Sack and Lameu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell viability assays and P2X7 receptor-induced pore formation in BK-treated neuroblastoma cell lines. Neuroblastoma cell lines (A) SH-5YSY; (B) IMR-32, and (C) CHP-100 exposed to different treatments for 24 h were screened for induction of cell death. Control measurements (without treatment) were normalized to 100%. Pore formation was determined as membrane permeability for ethidium bromide (20 μM) was measured by flow cytometry in (D) SH-5Y5Y, (E) IMR-32, and (F) CHP-100 cells. The cells had been pre-treated with BK (10 nM) for 24 h and were then incubated for 2, 5, 10, and 15 min with Bz-ATP (100 μM). Cells treated only with Bz-ATP and ethidium bromide served as control. Data are presented as mean values ± SD of three independent experiments.