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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Jan 10;553(7687):165–170. doi: 10.1038/nature25192

Extended Data Fig. 7. NLG-1 expression in multiple male sex muscles rescues nlg-1 mutant DVB neurite phenotype. Silencing spicule circuit or exposure to exogenous GABA does not reduce DVB neurites in nlg-1 mutant males.

Extended Data Fig. 7

(a) Confocal images of DVB (lim-6int4::wCherry), and quantification of (b) total neurite outgrowth and (c) number of neurite junctions in control, nlg-1(ok259), and nlg-1(ok259); nlg-1p::nlg-1::gfp, and nlg-1p::nlg-1::gfp day 3 males. Quantification of (d) total neurite outgrowth and (e) number of neurite junctions in control or nlg-1(ok259) mutant males with or without NLG-1 tissue-specific expression. Expression patterns for rescue promoters - lim-6int4 – DVB, gar-3b – SPC, spicule protractor muscles; unc-103F – SPC, PCA, PCB, other neurons; unc-103E – male sex muscles; flp-13 – spicule retractor muscles, CP6. (f) Confocal images of lim-6int4::wCherry and Ex[gar-3b::HisCl1::gfp], (g) total neurite length, and (h) number of neurite junctions of nlg-1(ok259) +/− 10mM histamine overnight, nlg-1(ok259); gar-3b::HisCl1::gfp +/− 10mM histamine overnight, and nlg-1(ok259) + 30mM GABA overnight in day 5 males. (dot=one animal, magenta bar=median, and boxes=quartiles, one-way ANOVA and post-hoc Tukey HSD).