Skip to main content
. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Jan 10;553(7687):165–170. doi: 10.1038/nature25192

Extended Data Fig. 10. DVB in hermaphrodites does not show neurite branching upon gar-3b::ChR2::yfp activation or NRX-1/NLG-1 manipulations.

Extended Data Fig. 10

(a) Confocal images of lim-6int4::wCherry and Ex[gar-3b::ChR2::yfp] expression in day 1 hermaphrodites showing DVB axon projection after activation on retinal (488 nm light for 3×15s every 45 mins for 4.5h). (b) Confocal images of lim-6int4::wCherry or lim-6int4::gfp in control, nrx-1(wy778), nlg-1(ok259), and Ex[lim-6int4::gfp::nrx-1LONG] hermaphrodites at day 3. (c) Quantification of the percentage of hermaphrodites with DVB axon abnormalities, neurites (in almost all cases, a single neurite off axon just posterior of the pre-anal ganglion) in day 1 control and Ex[gar-3b::ChR2::yfp] with activation, day 3 control, nrx-1(wy778), nlg-1(ok259), and Ex[lim-6int4::gfp::nrx-1LONG]. (number of worms indicated with n=, data points represent average percentage for each replicate of multiple hermaphrodites). (dot=one animal, magenta bar=median, and boxes=quartiles, one-way ANOVA and post-hoc Tukey HSD).