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. Author manuscript; available in PMC: 2018 Jul 10.
Published in final edited form as: Nature. 2018 Jan 10;553(7687):165–170. doi: 10.1038/nature25192

Extended Data Fig. 3. DVB inhibits expulsion-associated spicule protraction at day 3. Laser ablation of DVB and Channelrhodopsin expression in DVB and spicule protraction circuit.

Extended Data Fig. 3

(a) Confocal images of male worm with lim-6int4::wCherry and lim-6int4::HisCl1::gfp at day 3 and (b) quantification of the percentage of expulsion steps with spicule protraction for day 1 control, day 3 control, day 3 control + histamine, and day 3 lim-6int4::HisCl1::gfp + histamine males. (c) Quantification of time between consecutive expulsion steps for day 1 control, day 3 control, day 3 control + histamine, and day 3 lim-6int4::HisCl1::gfp + histamine males (+ histamine = 10mM histamine plates)(dot=one animal, magenta bar=median, and boxes=quartiles, one-way ANOVA and post-hoc Tukey HSD). (d) Confocal images of male worms with or without laser ablation of DVB at day 1–2, visualized with lim-6int4::gfp. (e) Confocal images of DVB (lim-6int4::wCherry) expressing channelrhodopsin at day 1 and 5, Ex[lim-6int4::ChR2::yfp]. (f) Confocal images of DVB (lim-6int4::wCherry) and spicule circuit expressing channelrhodopsin at day 1 and 5, Ex[gar-3b::ChR2::yfp]. (n>10 for d, e, and f).