Fig. 3.
Brain levels of Tris-buffered saline (TBS) and formic acid (FA) soluble amyloid-β (Aβ). a Levels of soluble Aβ aggregates, measured with 82E1 homogeneous enzyme-linked immunosorbent assay in TBS brain extracts centrifuged at 100,000 × g. Soluble Aβ aggregates were reduced by 40% in animals dosed with RmAb158-scFv8D3 (32 nmol/kg) and a tenfold higher dose of RmAb158 (320 nmol/kg), whereas the low dose of RmAb158 (32 nmol/kg) had no effect, compared with PBS-treated animals. b No significant reduction of soluble Aβ aggregates was seen in TBS brain extracts centrifuged at 16,000 × g. Total Aβ1–40 (c) and Aβ1–42 (d) in FA soluble brain extract revealed no significant differences between the treatment groups. e Aβ burden, determined with Aβ40 IHC, showed no significant differences between the groups in either cortex or hippocampus, although the interindividual variation was high. f Representative images from (e) with magnification of the brain region marked with a square. All graphs display scatterplots with bars representing group mean and error bars for SD. *** p < 0.001 and by two-way analysis of variance followed by Bonferroni’s post hoc test. n.s. nonsignificant