Fig. 7. DCEK-SIGN-R1 cells pretreated with 50 μM PF1 were incubated with 50 μg mL^–1 of each glycoconjugate or S. cerevisiae mannan for 2 h. Untreated cells were used as a negative control. After washing, images were obtained by using a confocal microscope. The nucleus of cells was stained with 1 μM Hoechst 33342 (scale bar: 10 μm). Graph shows quantitative fluorescence intensity (arbitrary units) of PF1 in treated cells (error bar: mean ± s.d., n = 3).