Fig. 6.

PPAR-γ was involved in the protective effect of AQP4 knockdown on BBB integrity via anti-inflammation. a PPAR-γ expression in astrocytes was quantified via Western blot. b Quantification of PPAR-γ. **p < 0.01 vs. the control + 5.5 mM glucose group, ^#p < 0.05 vs. the control + 0 mM glucose group. c The validity of the inhibitor GW9662 against PPAR-γ was confirmed via Western blot. d GW9662 significantly reduced claudin-5 expression in the co-culture models. e Quantification of PPAR-γ expression. **p < 0.01 vs. the si-AQP4 + 5.5 mM glucose group, ^##p < 0.01 vs. the si-AQP4 + 0 mM glucose group. f Quantification of claudin-5 expression. ^##p < 0.01 vs. the si-AQP4 + 0 mM glucose group. g TNF-α levels in the co-culture media from astrocytes as measured by ELISA. h IL-1β levels in the co-culture media from astrocytes as measured by ELISA. i IL-6 levels in the co-culture media from astrocytes as measured by ELISA. *p < 0.05 or **p < 0.01 vs. the control + 5.5 mM glucose group, ^#p < 0.05 vs. the control + 0 mM glucose group, ^{^}p < 0.05 vs. the si-AQP4 + 0 mM glucose group. n = 3 in each group