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. 2018 May 18;9:658. doi: 10.3389/fpls.2018.00658

FIGURE 2.

FIGURE 2

Defects in storage protein trafficking for the mag2-1, mip3-1 and mag2-1 mip3-1 mutants. (A,B) Immunoblot of dry seeds of wild-type and the mutants with anti-12S and anti-p2S antibodies. Arrows indicate SSP precursors. p12S, pro12S globulin; p2S, pro2S albumin; 12S, 12S globulin; 2S, 2S albumin. (C) Seed protein profile. Coomassie brilliant blue (CBB) staining of an SDS–PAGE gel for seed proteins. Abnormally accumulated SSP precursors are indicated by arrows. (D) Seed grains of wild type and the mutants. (E) Thousand-grain-weight (TGW) for wild type and the mutant seeds. (F) Protein content of one thousand grains is measured by BCA Protein Quantification Kit (ml410021, Mlbio, China).