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. 2018 May 18;9:658. doi: 10.3389/fpls.2018.00658

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Copyright © 2018 Zhao, Guo, Tang, Zhang, Wang, Kong, Zhang, Zhao, Lv and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ER stress is induced in the mutants. (A) Immunoblot of dry seeds of wild type, mag2-1, mip3-1 and mag2-1 mip3-1, with anti-BiP1/2 antibodies. Statistics for BiP1/2 protein accumulation in seeds are shown in numbers below the bands BiP/control (ACT or TUB, actin or tubulin) band concentration, measured by ImageJ, the same below). Three independent experiments per sample. (B) BiP3 expression was detected by RT-PCR on 9-day-old seedlings using BiP3 specific primers. Statistics for BIP3 expression level are shown in numbers below the bands. n = 3. Three independent experiments per sample. (C) Quantitative analysis of germination ratio (Green cotyledon ratio, the same below) of wild type, mag2-1, mip3-1 and mag2-1 mip3-1 on 1/2MS medium (left) or 1/2MS medium with 1.2 mM DTT (right). n = 72, three independent experiments per sample. (D) Root length change fold (treated/non-treated) of 7-old-seedlings of wild type, mag2-1 mip3-1 and mip2-1 mip3-1 on 1/2MS medium (non-treated) and 1/2MS medium with 0.15 mM DTT (treated). (E) Immunoblot on DTT treated 7-day-old seedlings of wild type, mag2-1, mip3-1 and mag2-1 mip3-1 on 1/2MS medium (left panel) or 1/2MS medium with 1.2 mM DTT (right panel) with anti-BiP1/2 antibody. Statistics of BiP1/2 protein level are shown below the bands. Change fold (treated/non-treated, the same below) is indicated in the lowest panel.