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Mutant seeds have lower protein carbonylation. (A) Quantification of seed protein carbonylation of wild type and mutants. (B) Carbonylated proteins were detected by immunodetection of protein-bound DNP after derivatization with hydrazine with anti-Dinitrophenol antibody (left panel). Right panel is CBB staining of a SDS–PAGE gel for total protein extracts from wild type and mutant seeds. Statistics of the bands are shown in numbers below the bands.
