Table 1.
Primers and probes used for PCR targets in the in-house M59 multiplex master mix
| Primers and probes | Sequence (5′– 3′)a | Reference |
|---|---|---|
| fliD-F | TGGTGACAGAGTGTATGATAATGGAA | Hojgaard et al. 2014 |
| fliD-R | ACTCCTCCGGAAGCCACAA | Hojgaard et al. 2014 |
| fliD-probe | FAM-TGCTAAAATGCTAGGAGATTGTCTGTCGCC-BHQ1 | Hojgaard et al. 2014 |
| 23S-F | TCGGTGAAATTGAAGTATC | Johnson et al. 2017 |
| 23S-R | CARGCTATAGTAAAGGTTCA | Johnson et al. 2017 |
| 23S-probe | HEX-CGTCTAACCACAAGTAATCGGCATC-BHQ1 | Johnson et al. 2017 |
| mIGS-F | TGTCGTTATCGGTATGTG | Johnson et al. 2017 |
| mIGS-R | AAGGGCCATGATGATTTG | Johnson et al. 2017 |
| mIGS-probe | Quas705-CCTTCCTACGACTTATCACCGACAG-BHQ3 | Johnson et al. 2017 |
| actin-F | GCCCTGGACTCCGAGCAG | Hojgaard et al. 2014 |
| actin-R | CCGTCGGGAAGCTCGTAGG | Hojgaard et al. 2014 |
| actin-probe | Q670-CCACCGCCGCCTCCTCTTCTTCC -BHQ3 | Hojgaard et al. 2014 |
a
BHQ1, BHQ3: Black Hole Quencher 1 and 3, respectively; FAM: 6-carboxyfluorescein; HEX: hexachlorofluorescein phosphramidite; Q670: Quasar 670; Q705: Quasar 705.