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. 2018 Apr 22;9(10):1821–1835. doi: 10.7150/jca.24934

Figure 2.

Figure 2

PAK7 promotes proliferation of breast cancer cells, and inhibits cell apoptosis. A, The cell were transfected with flag-PAK7 overexpression plasmid or flag-NC negative control vector, and small interfering RNA of PAK7 (siR-PAK7) or negative control (siR-NC) were detected by RT-qPCR and western blotting in MCF-7 and MDA-MB-231 cells. B, PAK7 overexpression enhances the proliferation of MCF-7 cells which was detected by the CCK-8 assay. C, PAK7 knockdown inhibits the proliferation of MDA-MB-231 cells which was detected by the CCK-8 assay. D, PAK7 overexpression enhances the proliferation of MCF-7 cells which was detected by the colon formation assay. E, PAK7 knockdown inhibits the proliferation of MDA-MB-231 cells which was detected by the colon formation assay. F, PAK7 overexpression increases the portion of MCF-7 cells in S and G2/M phase which was detected by flow cytometry. G, PAK7 knockdown increases the portion of MDA-MB-231 cells in G1 phase, and decreases the portion of cells in S and G2/M phase which was detected by flow cytometry. H, PAK7 overexpression inhibits early apoptosis and late apoptosis of MCF-7 cells which was detected by flow cytometry. I, PAK7 knockdown promotes early apoptosis and late apoptosis of MDA-MB-231 cells which was detected by flow cytometry. Values represent the mean ± SD from three independent measurements. *P < 0.05, **P < 0.01, ***P < 0.001.