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. 2018 Apr 26;9(10):1856–1862. doi: 10.7150/jca.24619

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CircHIPK3 overexpression suppressed cell proliferation, migration and invasion of OS cells in vitro. (A) qRT-PCR analysis of the effect on overexpression of circHIPK3 by vector transfection in the U2OS and 143B cell lines. (B) CCK-8 assays were performed to examine the cell proliferation rate of U2OS and 143B cells after circHIPK3 overexpression. (C) Clone formation assays were performed to examine cell vitality after transfection. (D) Transwell assays were performed to identify the capacity of cell invasion after transfection. (E) Wound healing assays were performed to examine the capacity of cell migration after transfection.*P < 0.05; **P < 0.01.