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. Author manuscript; available in PMC: 2018 May 25.
Published in final edited form as: Nat Biomed Eng. 2017 Oct 2;1:889–901. doi: 10.1038/s41551-017-0137-2

Figure 1. CRISPR-Gold can deliver Cas9 ribonucleoprotein and donor DNA in vivo and induce homology directed DNA (HDR) repair.

Figure 1

a) CRISPR-Gold is composed of 15 nm gold nanoparticles conjugated to thiol modified oligonucleotides, which are hybridized with donor ssODN and subsequently complexed with Cas9 RNP, and the endosomal disruptive polymer PAsp(DET).

b) CRISPR-Gold is internalized by cells in vitro and in vivo via endocytosis, triggers endosomal disruption, and releases Cas9 RNP and donor DNA into the cytoplasm. Nuclear delivery results in HDR.