Skip to main content
. Author manuscript; available in PMC: 2018 May 25.
Published in final edited form as: Nat Biomed Eng. 2017 Oct 2;1:889–901. doi: 10.1038/s41551-017-0137-2

Figure 5. CRISPR-Gold induces gene editing in the muscle tissue of Ai9 mice.

Figure 5

a) CRISPR-Gold was injected into Ai9 mice and gene deletion of the stop sequence in the Ai9 gene was determined by tdTomato expression.

b) Representative images of tdTomato fluorescence in the gastrocnemius muscle after a single CRISPR-Gold injection. A plasmid expressing Cre recombinase was injected with lipofectamine as a positive control. Scale bar is 500 µm.

c) tdTomato expression is observed in a broad area of the muscle after injection with CRISPR-Gold. The entire TA muscle cross-section image shows tdTomato expression after CRISPR-Gold injection. Scale bar is 500 µm.