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. Author manuscript; available in PMC: 2018 May 25.

Published in final edited form as: Nat Biomed Eng. 2017 Oct 2;1:889–901. doi: 10.1038/s41551-017-0137-2

Table 5.

sgRNA T7 Template Forward Primer

CXCR4	GATCCTAATACGACTCACTATAGAAGCGTGATGACAAAGAGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
Mdx	GATCCTAATACGACTCACTATAGGTCTTTGAAAGAGCAATAAAAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
GFP-L2	GATCCTAATACGACTCACTATAGCTGAAGCACTGCACGCCATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
CXCR4-F1 (Cas9-nickase)	GATCCTAATACGACTCACTATAGTACAGGCTGCACCTGTCAGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
DMD	GATCCTAATACGACTCACTATAGTCTTTGAAAGAGCAACAAAAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
Ai9-F	GGATCCTAATACGACTCACTATAgAAAGAATTGATTTGATACCGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC
Ai9-R	GGATCCTAATACGACTCACTATAgGTATGCTATACGAAGTTATTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC

gRNA primers
T7Rev-Long	AAAAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC
T7 Forward Primer : GGATCCTAATACGACTCACTATAG
T7 Reverse Primer : AAAAAAGCACCGACTCGG

Ai9 sequence was designed based on Tabebordbar et al.¹⁸