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. 2018 Apr 5;14(5):508–517. doi: 10.7150/ijbs.23318

Figure 4.

Figure 4

Effect of deletion of Chk2 on the proliferation and differentiation of BM-MSCs in Pthrp KI mice. Primary bone marrow cells from 2-week-old WT, Chk2-/-, Pthrp KI, and Chk2-/- KI mice were cultured ex vivo in osteogenic differentiation medium for 18 days and resulting cultures were stained with (A) methylene blue for total number of colonies (CFU-F) and (B) cytochemically for ALP to show CFU-Fap. (C) Total CFU-f-positive areas and (D) ALP-positive areas relative to culture dish area. (E) Relative levels of osteoblast- related genes. Each value is the mean±SEM of determinations in 5 mice of each group. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with WT mice; ## p < 0.01 compared with Pthrp KI mice.