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. 2018 Apr 25;14(5):577–585. doi: 10.7150/ijbs.22220

Figure 3.

Figure 3

STAT3 protects HCC cells from sorafenib induced cell death through regulating Mcl-1 expression. (A) The expression levels of STAT3, Mcl-1 and GAPDH in Hep3B, Huh7 and HepG2 were determined by Western Blot using anti-STAT3, anti-Mcl-1 and anti-GAPDH antibodies, respectively. (B) STAT3 was knocked down in Huh7 cells. The expression levels of Mcl-1 and STAT3 levels were determined. (C) STAT3 was over-expressed in Hep3B cells. The expression levels of Mcl-1 and STAT3 were determined. (D, E) STAT3 was knocked down in Huh7 cells. Cells were then treated with different doses of sorafenib for 24hours. The expression levels of Mcl-1, STAT3 and its phosphorylation levels were determined (D). The ratio of pSTAT3 to STAT3 was quantified using Image J (E).