Figure 2.

Expression of different hepatic genes by definitive endoderm (DE) cells cultured and differentiated under different conditions. Data are given for each individual sample to appreciate the variation within each condition and the overlap between the different conditions. Data are presented as Ct values of the respective genes normalized to Ct values of the housekeeping gene CREBBP. Results are from four independent differentiation experiments and seven donors. Due to poor RNA yield, some genes where only analysed in two (P‐gp) or three (CK7, BSEP, and BGP) of the cultures. PCLS = precision‐cut liver slices; PDMS = polydimethylsiloxane; PS = polystyrene [Colour figure can be viewed at http://wileyonlinelibrary.com]