Fig. 4.

Measurements of intracellular Ca²⁺ transients in single cardiomyocytes. Cardiomyocytes were isolated from the hearts of the septic rats euthanized at 4 h after LPS treatment. a Baseline Ca²⁺ concentration (baseline FFI). b Peak Ca²⁺ concentration during cell shortening (peak FFI). c Amplitude of Ca²⁺ transient (delta FFI). d Time to 90% Ca²⁺ decay (TP₉₀). e Time constant of Ca²⁺ decay (Tau). f Representative traces of cellular calcium transient in rat LV myocytes paced at 1 Hz. n = 88 from 5 LPS-treated mice; n = 35 from 5 control mice. ** p < 0.01 compared to the control.