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. 2018 Mar 2;93(4):436–447. doi: 10.1002/cyto.a.23348

Figure 1.

Figure 1

Presence of a CD14+ subpopulation of HIV‐1 gp120‐coated CEM.NKRCCR5 target cells in the ADCC‐GTL assay. (A) Gating strategy used for analysis of ADCC activity and evaluation of CD14 on the surface of SF162 gp120‐coated cells targeted for ADCC in ADCC‐GTL assays conducted with plasma from a chronically infected HIV‐seropositive donor, (B) plasma from an HIV‐1 virus controller, and (C) the HIV‐1 gp120 C1 region‐specific mAb C11. Histograms in the far right panels indicate the detection of CD14 on the surface of GzB+ target cells. (D) No CD14 was observed on the surface of GzB− target cells. (E) The RSV‐specific mAb Palivizumab was used as a negative control; as expected the frequency of GzB+ events was low and very few CD14+ cells were identified.