Figure 2.

Application of Area Scaling Analysis (ASA) to the ADCC‐GTL assay. (A) Gating strategy used for ASA of GzB⁺ SF162 gp120‐coated target cells in the modified ADCC‐GTL assay. This gating strategy was used to identify singlet (red gate) and non‐singlet (green gate) GzB⁺ target cell events in ADCC‐GTL assays performed with whole PBMC, PBMC depleted of CD14⁺ cells, PBMC depleted of CD56⁺ cells, and control PBMC depleted with biotin as sources of effector cells. ADCC antibody samples were plasma collected from an HIV‐1 infected donor during chronic infection (B), plasma from an HIV‐1 virus controller (C), and the C11 mAb (D). Data in the line graphs represents mean and SD from 3 independent experiments, and flow cytometry dot plots (B–D) include concatenated data from all 3 experiments.