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. 2018 Mar 26;47(9):1033–1042. doi: 10.1111/ejn.13901

Figure 2.

Figure 2

LUCID and TDE60% treatments improve spatial resolution in confocal images of fixed brains. (a) Representative apical dendrites of layer V pyramidal neurons (L5PNs) in confocal images of LUCID‐ (above), TDE60%‐ (middle), and non‐ (bottom) treated slices of Thy1‐EYFP‐H mice. Vertical bar indicates the color code. Scale bars, 2 μm. (b, c) Embedded 100‐nm yellow‐green (YG) beads in brain slices. XZ images of the Z‐stack (b). Magnified images of YG beads in regions i–xi are shown in XY and XZ images (c). Dashed lines show how fluorescence intensities were acquired along XY and XZ images. White scale bars indicate 200 nm. Yellow scale bar indicates 1 μm in the axial image of panel viii only. (d, e) Full width at half maximum (FWHM) of 100 nm YG beads (n = 10 each) at each depth: lateral FWHMs in XY images (d) and axial FWHMs in XZ images (e) in individual brain slices. All figures were maximum‐intensity projection images. [Colour figure can be viewed at http://www.wileyonlinelibrary.com/].