Table 1.

Metal content and thermal melting analyses of MnSOD‐3 and MnSOD‐3[Q142H] cultured in minimal media containing either iron or manganese.

Purified protein[a]	Media	Manganese[b]	Iron[b]	T m [°C][c]
MnSOD‐3	Mn	1.02±0.051	0.01±0.001	73
MnSOD‐3	Fe	0.13±0.006	0.92±0.048	84
MnSOD‐3[Q142H]	Mn	0.63±0.031	0.20±0.010	68
MnSOD‐3[Q142H]	Fe	0.02±0.001	0.93±0.046	66

[a] Proteins induced from the appropriate plasmid in E.coli SOD‐deficient cells. [b] Metal content indicated in g atoms per subunit. Errors were consistently approx. 5 %. [c] Thermal melting temperatures determined by CD.