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. 2018 Feb 20;33(3):224–233. doi: 10.1111/omi.12215

Figure 4.

Figure 4

In vitro translation of dihydrofolate reductase (DHFR) in the presence of synthetic peptides representing the toxin and antitoxin proteins of the D11S_1718‐1719 and D11S_1194‐1195 toxin‐antitoxin (TA) systems. In vitro translation reactions were incubated with the toxin or antitoxin proteins of the TA systems, either alone or in combination and reaction products were electrophoresed in a Tris‐Glycine SDS‐PAGE gel. Lanes 1 and 8, positive control reaction without toxin or antitoxin protein (arrows indicates the DHFR protein); Lane 2, D11S_1194 toxin only; Lane 3, D11S_1195 antitoxin only; Lane 4, equal mixture of the D11S_1194‐1195 toxin and antitoxin; Lane 5, D11S_1718 toxin only; Lane 6, D11S_1719 antitoxin only; Lane 7, equal mixture of the D11S_1718‐1719 toxin and antitoxin; Lane 9, empty; Lane 10, size markers