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Extent of DNA‐duplex formation under different MgCl2 concentrations (0–5 mm) using ODNs with four complementary bases. A) Change in Cy3/Cy5 FRET signals upon duplex formation with complementary unmodified ODNs (0.5 μm). B) Significant change in Cy3/Cy5 FRET signals upon duplex formation with complementary ODN‐BiPy‐Disc 3 e and 3 j (0.5 μm). C) Cy3/Cy5 FRET signals with ODN‐BiPy‐Disc (3 i) lacking of complementary bases.
