Table 3.
Primary antibodies used for dot blotting and Western blotting
| Protein | Primary antibody and supplier | Host species and isotype (antibody type) | Concentration | Molecular mass (kDa) |
|---|---|---|---|---|
| HSP27 | Thermo Fisher Scientific (#MA3‐015) | Mouse IgG (monoclonal) | 1:1000 | ~27 |
| p‐ACC Ser79 | Cell Signaling Technology (#3661S) | Rabbit IgG (polyclonal) | 1:1000 | ~257 |
| ACC | Cell Signaling Technology (#3676S) | Rabbit IgG (monoclonal) | 1:1000 | ~257 |
| p‐AMPK‐α Thr172 | Cell Signaling Technology (#2535) | Rabbit IgG (monoclonal) | 1:500 | ~63 |
| AMPK‐α | Cell Signaling Technology (#2603) | Rabbit IgG (monoclonal) | 1:1000 | ~63 |
| p‐CaMKII Thr287 | Cell Signaling Technology (#12716) | Rabbit IgG (monoclonal) | 1:1000 | ~50‐80 |
| CaMKII | Cell Signaling Technology (#4436) | Rabbit IgG (monoclonal) | 1:1000 | ~50‐80 |
| p‐PLB Ser16 | Merck Millipore (#07‐052) | Rabbit IgG (polyclonal) | 1:2000 | ~25 |
| PLB | Abcam (#ab2865) | Mouse IgG (monoclonal) | 1:1000 | ~25 |
| MHC I | Developmental Studies Hybridoma Bank, University of Iowa (#A4.840) | Mouse, IgM (monoclonal) | 1:200 | ~200 |
| MHC IIa | Developmental Studies Hybridoma Bank, University of Iowa (#A4.74) | Mouse, IgG (monoclonal) | 1:200 | ~200 |
| MHC IIx | Developmental Studies Hybridoma Bank, University of Iowa (#6H1) | Mouse, IgM (monoclonal) | 1:100 | ~200 |
Antibodies were diluted in 1% bovine serum albumin in 1× phosphate‐buffered saline with 0.02% sodium azide and 0.025% Tween.