Figure 3.

Neurogenesis in the SGZ of adult rats is unchanged at 9 weeks following DA or NA lesions, or combined double lesions. A representative image from a control animal showing immunohistochemistry for BrdU and Prox1 at 4 weeks after the 7‐day BrdU pulse period that was used to quantify the number of neurons generated in that time (a–d). (b–d) represents a high magnification example of a BrdU+/Prox1+ cell. A total of 80 BrdU+ cells in the dentate SGZ were assessed for Prox1 immunoreactivity by orthogonal reconstruction of confocal images in order to determine the fractional contribution to BrdU+ cells (e). Statistical analysis: (e) One‐way ANOVA with Dunnett's test for multiple comparison, F _3,32 = 0.6291 and p = .6016. Error bars = Standard deviation. For control groups n = 16, for lesion groups n = 8. Scale bars: A 200 µm. Abbreviations: BrdU, 5‐bromo‐2′‐deoxyuridine; NA, noradrenaline; DA, dopamine; SGZ, subgranular zone [Color figure can be viewed at http://wileyonlinelibrary.com]