Figure 4.

Delphinidin inhibits cell migration via the MAPK‐signaling pathway. (A) Cell migration was determined using a wound healing assay after 24 h of treatment with 75 μM delphinidin. The data are expressed as the mean ± SEM (n = 3). The statistical significance of each group was analyzed by a two‐way ANOVA test [***P < 0.001 at HOS and ###P < 0.001 at U2OS compared to the non‐treated group (0 μM)]. (B) The MAPK‐signaling pathway‐related protein levels were assayed with a western blot. β‐actin was used as an internal control