Table 1.
Genes | Primers (sequences 5'→3') | Product size (bp) |
rpoB | F (5′-ACGGTCGGCGAGCTGATCC-3′) R (5′-CAGACCGATGTTGGGCCCCT-3′) |
351 |
katG | F (5′-GACATTCGCGAGACGTTTCGG-3′) R (5′-GCTCTTAAGGCTGGCAATCTCG-3′) |
469 |
inhA | F (5′-CTATATCTCCGGTGCGGTCA-3′) R (5′-CTTGGCCATCGAAGCATAC-3′) |
469 |
rpsL | F (5′-AGGTCACGGCGTACATTCC-3′) R(5′-GCCCTTCTCCTTCTTAGCG-3′) |
188 |
embB | F (5′-TGATATTCGGCTTCCTGCTC-3′) R (5′-ACCGCTCGATCAGCACATAG-3′) |
417 |
embB, EMB mutant gene; katG and inhA, INH mutant gene; rpoB, RFP mutant gene; rpsL, SM mutant gene.