Figure 4.

Lactate dehydrogenase (LDH) activity in the medium of bile acid treated cells. McNtcp.24, CHO.asbt.35, McArdle RH-7777, and CHO cells were incubated in medium supplemented with bile acids (100 μM). (A) Glycine-conjugated bile acids, but not TCA, induced the time dependent release of LDH in the culture medium of McNtcp.24. (B) Both glycine- and taurine-conjugated bile acids induced the release of LDH from CHO.asbt.35 cells. Neither glycine- nor taurine-conjugated bile acids induced the release of LDH in McArdle RH-7777 (C) or CHO (D) cells after a 5 h incubation. The means ± SD (n = 3) are shown per time point. These experiments were repeated six times with similar results.