Table 3. Folded population of the reporter residues and their thermodynamic analysis deduced from NMR in acetate buffer (pH 3.8) at 25 °C.

	Fraction folded a (%)				% folded population	ΔGfold (kcal mol–1)
	Val3	Val5	Lys8	Ile10
2	39	60	36	38	43 ± 10	0.17 ± 0.2
3	68	80	68	59	69 ± 7	–0.48 ± 0.1
3b	38	63	32	30	41 ± 13	0.22 ± 0.2
3e	64	80	67	59	67 ± 8	–0.42 ± 0.2
3n	78	99	76	70	81 ± 11	–0.86 ± 0.3
4g	84	99	81	75	85 ± 9	–1.03 ± 0.3
5	95	99	89	85	92 ± 5	–1.44 ± 0.4
pG	81	87	83	76	82 ± 3	–0.90 ± 0.1

^aFraction folded and ΔG_fold were calculated from the respective fully folded disulfide bridged cyclic variant and an unfolded random coil by established methods (ESI).