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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol Methods. 2018 Apr 22;458:53–57. doi: 10.1016/j.jim.2018.04.006

Table 2.

Guidelines for different immune cell numbers for a 96-well microplate set-up.

Cell Type Cell # per well of a 96-well Biolog microplate
BMDCs (Murine Bone marrow-derived DCs) 50,000
Human monocyte-derived DCs 50,000 – 80,000
Lymphocytes – naïve or activated B and T cells 100,000