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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Neurobiol Dis. 2018 Apr 13;115:182–193. doi: 10.1016/j.nbd.2018.04.010

Fig. 2.

Fig. 2

CBX corrects GAP junction communication in MBECs. A, After calcium-AM was loaded into cells, whole cell FRAP analysis was used to investigate GAP junction communication in Cln3R and Cln3−/− MBECs. Cells were treated for 2 h with vehicle or 25 μM CBX. ImageJ was used to quantify FRAP over time. Scale bar 20 μm. Data show mean ± s.e.m. from n = 76, 42, 52, 53 Cln3−/−, Cln3R, Cln3−/− + CBX, Cln3R + CBX cells respectively. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test. Relative to Cln3R, Cln3−/− MBECs had significantly more calcium-AM recovery at all-time points. * p < 0.05, ** p < 0.01, **** p < 0.0001. B, Plasma membrane enriched fractions were analyzed by western blot for Connexin 43 levels. Bars show mean ± s.e.m. of six independent experiments. Analysis with Kruskal-Wallis test showed no significant differences among experimental groups.