Figure 5.

CB002 induces expression of p53 target genes independently of p73 and NOXA is required for CB002-mediated apoptosis. A-(C)Whole cell lysates were subjected to western blot analysis A. p73 was knocked-down by siRNA in SW480 cells followed by CB002 treatment of 16 hrs. B. DR5 was knocked-down by siRNA in DLD-1 cells followed by CB002 treatment of 16 hrs. C. NOXA was knocked-down by shRNA in SW480 cells followed by CB002 treatment of 16 hrs. D-E. Parental SW480 and SW480 NOXA stable knockdown cells treated with CB002 for 48 hrs were subjected to a sub-G1 analysis. E. Two-way ANOVA statistical analysis was performed for results from panel D, p ≤ 0.0001 against DMSO vehicle control. Three replicates were performed, and a representative histogram is shown. c-caspase 3 corresponds to the cleaved form of full-length caspase-3.