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CB002 does not reduce mutant p53 stability in SW480 and DLD-1 cells. A-B. Experiments with SW480 cells. C. Analysis of DLD-1 colorectal cancer cells. Cells were treated for a 24 hr period with DMSO, CB002 or positive control followed by 100 μg/mL cycloheximide addition, and protein stability was evaluated in a time course ranging from 0 - 10 hrs. Histone deacetylase inhibitor, SAHA and Hsp90 inhibitor Geldanamycin (1 μM GA) were used as positive controls.
